H3R8 and H4R3 are the key repressive histone methylation sites that are methylated by PRMT5 ( 18, 19). Protein arginine methyltransferase 5 (PRMT5), a type II arginine methyltransferase, regulates gene transcription and modulates chromatin structure through symmetrically dimethylating arginine residues on target proteins ( 16, 17). SET8 has been reported to participate in diverse biological processes, the regulation of gene transcription, maintenance of genomic stability, formation of heterochromatin, and cell cycle progression and development ( 9– 15).
SET domain-containing protein 8 (SET8) is a member of the SET domain-containing methyltransferase family, which particularly catalyzes the monomethylation of histone H4 Lys20 ( 6– 8).
Histone methylation can be divided into arginine methylation and lysine methylation. Increasing evidence has suggested that tumor-associated histone methylation is an important determinant of initiation and the development of HCC, and represents a promising therapeutic target ( 5). Furthermore, the majority of epigenetic alterations are reversible, and therefore could provide a prospective treatment for HCC ( 4). Epigenetic modifications can affect gene expression at the cancer transcriptome level more extensively than genetic changes. The initiation and progression of hepatocellular carcinoma (HCC) is regarded as a multi-step process entailing genetic and epigenetic alterations ( 1– 3). The independent pattern of histone methylation represents a novel insight into tumor progression and therapeutic targets for HCC. In conclusion, high expression of SET8 combined with PRMT5 was associated with a high rate of recurrence and poor survival in patients with HCC. The combination of SET8 and PRMT5 demonstrated an improved capacity to predict patient mortality and disease recurrence (P=0.002 and P=0.004, respectively), particularly for the prediction of early recurrence (P<0.001). Multivariate Cox analysis revealed that SET8 and PRMT5, along with vascular invasion, tumor size and tumor number, were independent prognostic factors for OS and TTR. Immunohistochemical analysis demonstrated that high expression of SET8 and PRMT5 was significantly associated with poor overall survival (OS, P<0.001) and time to recurrence (TTR, P<0.001). Positive SET8 expression was noted in 104 patients (53.3%), and was associated with PRMT5 expression (n=106, 54.4%, P<0.05). Expression was analyzed for correlations with clinicopathological features, marker co-expression and patients' survival by univariate and multivariate analyses. Immunohistochemical staining for SET8 and PRMT5 was performed on paraffin-embedded tumor tissue microarrays. The retrospective study included 195 consecutive patients who had undergone hepatectomy for HCC. The aim of the present study was to investigate the association between histone methylation and HCC phenotype, and the prognostic value of combining expression levels of SET domain-containing protein 8 (SET8) with protein arginine methyltransferase 5 (PRMT5) in patients with HCC following curative resection. However, whether the expression profile of multiple histone methyltransferases represents a poorer prognosis is entirely unknown.
MEDIA CYBERNETICS IMAGE PRO PLUS PRICE SOFTWARE
Image-Pro Plus is the image analysis software used daily by thousands of researchers and engineers in medicine, science, and industry.Histone methyltransferases are important determinants of the initiation and progression of hepatocellular carcinoma (HCC) and represent promising therapeutic targets. The company’s products simplify and enhance image-based data collection and analysis to increase the accuracy and productivity of its customers. įounded in 1981, Media Cybernetics develops image informatics solutions to automate research, development, and quality control processes in life science and industry. To find out more about the Aexeon Video Capture Kit, go to.
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It comes complete with an Aexeon board, video interface cable, and install disk with Aexeon and ImagePro drivers. The Aexeon Video Capture Kit supports analog cameras and is the ideal solution for anyone involved in electronic imaging who wants to simplify the purchase and integration of imaging software and hardware components. Media Cybernetics have also annonced that it has released the new Aexeon Video Capture Kit for Image-Pro Plus 7. , Indianpolis, IN - dPict Imaging today annouced support Image Pro Plus 7 by Media Cybernetics for its Aexeon line of products. DPict Adds Support for Media Cybernetics Image Pro Plus 7 Support to Include Aexeon Product Line
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